L-PHENYLALANINE

Quality Standards: USP24, FCCIV
Preserve in well- closed containers, at controlled room temperature.
Recommended limit: 2 year.
Characteristics: White crystals or crystalline powder; slightly bitter taste. Feely soluble in formic acid, sparingly soluble in water. It can be used safely in any food or beverage. In addition to it's major use as the main material for aspartame, it is also used for the synthesis of anti-cancer and antivirus medicines.
 

Chemical Formula	C9H11O2N
Molecular Weight	165.19
[Compositional Specifications of L-Phenylalanine]
Content	L-Phenylalanine. when calculated on the dried basis. contains 98.5~102.0% of L-phenylalanine (C9H11O2N).
Description	L-Phenylalanine occurs as white crystals or crystalline powder and has a slightly bitter taste.
Identification	(1) To 5 ml of L-Phenylalanine solution (1→1000), add 1 ml of potassium dichromate solution (1→1000).  Upon heating the solution, characteristic scent is generated. (2) To 5 ml of L-Phenylalanine solution (1→1.000), add 1 ml of ninhydrin solution (1→1,000), and heat for 3 minutes. A purple color becomes. (3) To 10 mg of L-Phenylalanine, add 0.5 g of potassium nitrate and 2 ml of sulfuric acid. heat in a water bath for 20 minutes. After cooling, add 5 ml of hydroxylamine hydrochloride solution (1→10), set aside in ice water for 10 minutes. Add 8 ml of 40% sodium hydroxide solution , and set aside. A red-purple color becomes.
Purity	(1) Clarity of Solution : When 1 g of L-Phenylalanine is dissolved in 100 ml of water, the solution should be colorless and almost clear. (2) pH : 1 g of L-Phenylalanine, dissolved in 100 ml of water. pH of this solution should be 5.4~6.0. (3) Specific rotation : After drying for 3 hours at 105°, approximately 1 g is precisely weighed and dissolved in 50 ml of water. Optical rotation of this solution should be [α](20, D) =-33.0∼-35.2° (4) Arsenic : 0.25 g of L-Phenylalanine is dissolved in 10 ml of 2 N hydrochloric acid, use the Test Solution. This Test Solution is proceed as directed under Arsenic, it's content should not be more than 4ppm. (5) Heavy metals : 1 g of L-Phenylalanine is dissolved in 2 ml of diluted acetic acid and 40 ml of water use the Test Solution. This Test Solution proceed as directed under heavy metals and its content should not be more than 20ppm. (6) Chloride : When 0.5 g of L-Phenylalanine proceed as directed under chloride, its content should not be more than the amount that corresponds to 0.3 ml of 0.01 N hydrochloric acid.
Loss on Drying	When L-Phenylalanine is dried for 3 hours at 105°, the weight loss should not be more than 0.3%.
Residue on Ignition	When thermogravimetric analysis is done with L-Phenylalanine, the residue should not be more than 0.1%.
Assay	Weigh accurately about 0.3 g of L-Phenylalanine, and proceed as directed under Assay in DL-Alanine.        0.l N perchloric acid1 ml  = 16.52 mg of C9H11O2N

 

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