L-ARGININE

Molecular Formula: C6H14N4O2
Molecular Weight: 174.20
Characteristics: White crystals or crystalline powder . Bitter taste. Very slightly soluble in water.
Quality Standards: EP5, AJI92, USP26, FCCIV
Mainly uses:
Used in food additive and nutritional aggrandizement.
Used in the curing of hepatic coma, the preparation of amino acid transfusion; or used in the injection of liver disease.
 

Chemical Formula	C6H14N4O2
Molecular Weight	174.20
[Compositional Specifications of L-Arginine]
Content	If L-Arginine is converted to a dried form, it should contain 98.0∼102.0%  L-arginine (C6H14N4O2).
Description	L-Arginine is white crystallite or crystalline powder with unique scent and taste.
Identification	(1) 1 ml of ninhydrine solution (1→50) is added to 5 ml aqueous solution of L-Arginine (1→1,000). Upon heating for 3 minutes in a water bath, this solution turns violet. (2) Aqueous solution of L-Arginine is alkaline.
Purity	(1) Clarity of Solution : When 1 g of L-Arginine is dissolved in 20 ml of water, the solution should be colorless and clear. (2) pH : pH of aqueous solution (1→20) of L-Arginine should be in a range of 10.5∼12.5. (3) Specific Rotation : Approximately 8 g of L-Arginine is accurately weighed, which is dissolved in 6 N hydrochloric acid so that the total volume becomes 100 ml. Optical rotation of the solution is measured. When it is translated to dried material, α(20, D) = +25.0∼+27.9°. (4) Chlorides : When 0.07 g of L-Arginine tested for chlorides, the detected amount should not be more than to the chloride content of 0.2 ml of 0.01 N hydrochloric acid. (5) Arsenic : 0.25 g of L-Arginine is dissolved in 5 ml of water. When test for arsenic is carried out with this test solution, it should not be more than 4ppm. (6) Heavy Metals : 1 g of L-Arginine is dissolved in 30 ml of water, where 1 drop of phenolphthalein is added. This solution is neutralized with dilute hydrochloric acid. Test solution is prepared by further adding 2 ml of diluted acetic acid (1→20) and water so that the total volume of the solution becomes 50 ml. The content of heavy metals should not be more than 20ppm.
Loss on Drying	When L-Arginine is dried for 3 hours at 105°, the weight loss should not be more than 1.0%.
Residue on Ignition	Residue after ignition should not be more than 0.2%.
Assay	Approximately 0.2 g is accurately weighed and dissolved in 3 ml of formic acid, where glacial acetic acid (for non-aqueous titration) is added to bring the total volume to 50 ml. This solution is titrated with 0.1 N perchloric acid (indicator : 1 ml of crystal violet buffered in glacial acetic acid). At the end point, solution turns from pale violet to blue, then to green. Separately, a blank experiment is done following the same procedure.      0.1 N perchloric acid solution 1 ml = 8.710 mg C6H14N4O2

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Category: L-ARGININE